Journal: Gels

Article Title: A 3D Collagen–Alginate Hydrogel Model for Mechanoregulation of Autophagy in Periodontal Ligament Cells

doi: 10.3390/gels12010091

Figure Lengend Snippet: 3D compression promotes autophagy of PDLCs. ( A ) qRT-PCR analysis for quantification of ATG7 expression at the indicated time points. ( B ) qRT-PCR analysis for quantification of Beclin1 expression at the indicated time points. ( C ) Representative immunofluorescence images of LC3 staining at different time points. Scale bar: 100 μm. ( D ) Ratio of LC3-II/LC3-I quantified at different time points. ( E ) Immunoblotting analysis of P62 protein levels over time. ( F ) Densitometric quantification of P62 normalized to GAPDH. For ( A , B , D , F ), **, ***, ****, and ns indicate p < 0.01, p < 0.001, p < 0.0001 and no significant difference by one-way ANOVA, respectively. Shown as the mean ± SD.

Article Snippet: Samples were incubated with LC3 primary antibody (1:200) overnight at 4 °C, followed by secondary antibody and DAPI staining (Boster).

Techniques: Quantitative RT-PCR, Expressing, Immunofluorescence, Staining, Western Blot

Journal: Gels

Article Title: A 3D Collagen–Alginate Hydrogel Model for Mechanoregulation of Autophagy in Periodontal Ligament Cells

doi: 10.3390/gels12010091

Figure Lengend Snippet: AKT-mTOR signaling links compression to autophagy in PDLCs. Compression is transmitted through AKT-mTOR signaling. Modulation of this pathway by the AKT activator SC79 and the mTOR pathway modulator 3BDO alters autophagy-related gene expression. Changes in Beclin1, P62, ATG7, ATG5 and LC3-II reflect the formation of autolysosomes and the overall level of autophagic activity in periodontal ligament cells under compression. The light red arrows indicate the increase in the levels of AKT and mTOR.

Article Snippet: Samples were incubated with LC3 primary antibody (1:200) overnight at 4 °C, followed by secondary antibody and DAPI staining (Boster).

Techniques: Gene Expression, Activity Assay